MGP Database

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MGP003628

UniProt Annotations

Entry Information
Gene Namealdo-keto reductase family 1, member C3
Protein EntryAK1C3_HUMAN
UniProt IDP42330
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; IsoId=P42330-1; Sequence=Displayed; Name=2; IsoId=P42330-2; Sequence=VSP_055798; Note=No experimental confirmation available.;
Biophysicochemical PropertiesKinetic parameters: KM=142.1 uM for progesterone {ECO:0000269|PubMed:10557352}; KM=2.37 uM for 5-alpha-dihydrotestosterone {ECO:0000269|PubMed:10557352}; KM=1.0 uM for androstanediol {ECO:0000269|PubMed:10557352}; Vmax=20.1 nmol/min/mg enzyme with progesterone as substrate {ECO:0000269|PubMed:10557352}; Vmax=1.8 nmol/min/mg enzyme with 5-alpha-dihydrotestosterone as substrate {ECO:0000269|PubMed:10557352}; Vmax=4.4 nmol/min/mg enzyme with androstanediol as substrate {ECO:0000269|PubMed:10557352};
Catalytic Activity(5Z,13E)-(15S)-9-alpha,11-alpha,15- trihydroxyprosta-5,13-dienoate + NADP(+) = (5Z,13E)-(15S)-9- alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH.
Catalytic ActivityA 3-alpha-hydroxysteroid + NAD(P)(+) = a 3- oxosteroid + NAD(P)H.
Catalytic ActivityIndan-1-ol + NAD(P)(+) = indanone + NAD(P)H.
Catalytic ActivityTestosterone + NAD(+) = androstenedione + NADH.
Catalytic ActivityTestosterone + NADP(+) = androst-4-ene-3,17- dione + NADPH.
Catalytic ActivityTrans-1,2-dihydrobenzene-1,2-diol + NADP(+) = catechol + NADPH.
Enzyme RegulationStrongly inhibited by nonsteroidal anti- inflammatory drugs (NSAID) including flufenamic acid and indomethacin. Also inhibited by the flavinoid, rutin, and by selective serotonin inhibitors (SSRIs).
FunctionCatalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta- PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone.
Sequence CautionSequence=BAA04619.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
SimilarityBelongs to the aldo/keto reductase family. {ECO:0000305}.
Subcellular LocationCytoplasm.
Tissue SpecificityExpressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate. {ECO:0000269|PubMed:10557352, ECO:0000269|PubMed:10622721, ECO:0000269|PubMed:11165022, ECO:0000269|PubMed:7650035, ECO:0000269|PubMed:9415401, ECO:0000269|PubMed:9927279}.
Web ResourceName=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/AKR1C3ID612ch10p15.html";
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