Summary of Study ST002445

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001576. The data can be accessed directly via it's Project DOI: 10.21228/M80709 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002445
Study TitleThe interaction of ceramide-1-phosphate with group IVA cytosolic phospholipase A2 modulates neutrophil polarization during inflammatory responses
Study SummaryBone marrow-derived mouse neutrophils from wildtype, cPLA2alpha-knockin (interaction site on cPLA2alpha for its substrate C1P was ablated), and cPLA2alpha-knockout (cPLA2alpha gene mutated) mice were exposed to 4 hours of trans-endothelial migration and resulting eicosanoids were analyzed (eg, PGE2, PGD2, 5-HETE, and 5-oxo-ETE).
Institute
University of South Florida
Last NameMaus
First NameKenneth
Address4202 E Fowler Ave, CMMB - NES 107 - Chalfant Lab
Emailkmaus@usf.edu
Phone8139283137
Submit Date2023-01-16
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2023-02-05
Release Version1
Kenneth Maus Kenneth Maus
https://dx.doi.org/10.21228/M80709
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003983
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu Nexara X2 LC-30AD
Column MilliporeSigma Supelco Ascentis Express C18 (150 x 4.6 mm, 2.7 um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap
Ion Mode NEGATIVE
Units pmol/mL
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