Summary of Study ST002445
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001576. The data can be accessed directly via it's Project DOI: 10.21228/M80709 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002445 |
Study Title | The interaction of ceramide-1-phosphate with group IVA cytosolic phospholipase A2 modulates neutrophil polarization during inflammatory responses |
Study Summary | Bone marrow-derived mouse neutrophils from wildtype, cPLA2alpha-knockin (interaction site on cPLA2alpha for its substrate C1P was ablated), and cPLA2alpha-knockout (cPLA2alpha gene mutated) mice were exposed to 4 hours of trans-endothelial migration and resulting eicosanoids were analyzed (eg, PGE2, PGD2, 5-HETE, and 5-oxo-ETE). |
Institute | University of South Florida |
Last Name | Maus |
First Name | Kenneth |
Address | 4202 E Fowler Ave, CMMB - NES 107 - Chalfant Lab |
kmaus@usf.edu | |
Phone | 8139283137 |
Submit Date | 2023-01-16 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2023-02-05 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003983 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu Nexara X2 LC-30AD |
Column | MilliporeSigma Supelco Ascentis Express C18 (150 x 4.6 mm, 2.7 um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap |
Ion Mode | NEGATIVE |
Units | pmol/mL |
Chromatography:
Chromatography ID: | CH002945 |
Instrument Name: | Shimadzu Nexara X2 LC-30AD |
Column Name: | MilliporeSigma Supelco Ascentis Express C18 (150 x 4.6 mm, 2.7 um) |
Column Temperature: | 40 |
Flow Gradient: | The column was equilibrated with 100% Solvent A for 5 min and then 10 µl of sample was injected. 100% Solvent A was used for the first two minutes of elution. Solvent B was increased in a linear gradient to 25% Solvent B at 3 min, to 30% at 6 min., to 55% at 6.1 min, to 70% at 10 min, and to 100% at 10.10 min. 100% Solvent B was held constant until 13.0 min, where it was decreased to 0% Solvent B and 100% Solvent A from 13.0 min to 13.1 min. From 13.1 min to 14.0 min. Solvent A was held constant at 100% |
Flow Rate: | 0.5 ml/min |
Solvent A: | 20% acetonitrile/80% water; 0.02% formic acid |
Solvent B: | 20% acetonitrile/80% isopropanol; 0.02% formic acid |
Chromatography Type: | Reversed phase |