Summary of Study ST002445
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001576. The data can be accessed directly via it's Project DOI: 10.21228/M80709 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002445 |
| Study Title | The interaction of ceramide-1-phosphate with group IVA cytosolic phospholipase A2 modulates neutrophil polarization during inflammatory responses |
| Study Summary | Bone marrow-derived mouse neutrophils from wildtype, cPLA2alpha-knockin (interaction site on cPLA2alpha for its substrate C1P was ablated), and cPLA2alpha-knockout (cPLA2alpha gene mutated) mice were exposed to 4 hours of trans-endothelial migration and resulting eicosanoids were analyzed (eg, PGE2, PGD2, 5-HETE, and 5-oxo-ETE). |
| Institute | University of South Florida |
| Last Name | Maus |
| First Name | Kenneth |
| Address | 4202 E Fowler Ave, CMMB - NES 107 - Chalfant Lab |
| kmaus@usf.edu | |
| Phone | 8139283137 |
| Submit Date | 2023-01-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-05 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002527 |
| Collection Summary: | HUVECs were seeded on the upper wells Corning® Transwell® polycarbonate membrane inserts with 3.0 µm pores (Sigma-Aldrich CLS3415) inserted into a 24-well tissue culture plate at a density of 1.6 x 106 cells per ml in 250 µl volume. Inflammatory response was induced by adding TNFα (Cayman #32020, 20 ng/ml) 16-18 hours before addition of PNs. PNs were then isolated from mouse bone marrow and added to the upper well while HUVEC media containing lipopolysaccharides (LPS) from E. coli (Millipore Sigma #L3023, 1 µg/ml) was added to the bottom well as a chemoattractant. After time indicated (e.g., 4 hours), media and cells from the upper and lower wells were extracted, counted, combined, centrifuged, and washed in preparation for UHPLC-MS/MS. |
| Sample Type: | Bone marrow |
