Summary of Study ST002445
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001576. The data can be accessed directly via it's Project DOI: 10.21228/M80709 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002445 |
| Study Title | The interaction of ceramide-1-phosphate with group IVA cytosolic phospholipase A2 modulates neutrophil polarization during inflammatory responses |
| Study Summary | Bone marrow-derived mouse neutrophils from wildtype, cPLA2alpha-knockin (interaction site on cPLA2alpha for its substrate C1P was ablated), and cPLA2alpha-knockout (cPLA2alpha gene mutated) mice were exposed to 4 hours of trans-endothelial migration and resulting eicosanoids were analyzed (eg, PGE2, PGD2, 5-HETE, and 5-oxo-ETE). |
| Institute | University of South Florida |
| Last Name | Maus |
| First Name | Kenneth |
| Address | 4202 E Fowler Ave, CMMB - NES 107 - Chalfant Lab |
| kmaus@usf.edu | |
| Phone | 8139283137 |
| Submit Date | 2023-01-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003983 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu Nexara X2 LC-30AD |
| Column | MilliporeSigma Supelco Ascentis Express C18 (150 x 4.6 mm, 2.7 um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | pmol/mL |
MS:
| MS ID: | MS003717 |
| Analysis ID: | AN003983 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Eicosanoids were analyzed via mass spec using an AB Sciex Triple Quad 5500 Mass Spectrometer as previously described by Mitra et al., 2007 (FEBS Lett 581, 581–793). Q1 and Q3 were set to detect distinctive precursor and product ion pairs. Ions were fragmented in Q2 using N2 gas for collisionally induced dissociation. Analysis used multiple-reaction monitoring in negative-ion mode. Eicosanoids were monitored using precursor → product MRM pairs. The mass spectrometer parameters as previously described by Chalfant et al., 2001 and Wijesinghe et al., 2013 were: Curtain Gas: 20; CAD: Medium; Ion Spray Voltage: -4500V; Temperature: 300°C; Gas 1: 40; Gas 2: 60; Declustering Potential, Collision Energy, and Cell Exit Potential vary per transition |
| Ion Mode: | NEGATIVE |
