Summary of Study ST000246

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.


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Study IDST000246
Study TitleLipid Extraction Efficiency Comparison
Study TypeLipid Extraction Efficiency Comparison
Study SummaryAliquots of Jurkat T-lymphocyte cells were extracted using the Folch or Matyash method at different total volumes and run in triplicate to calculate the extraction efficiency for each method.
University of Florida
DepartmentDept. of Chemistry/SECIM
LaboratoryBiomedical Mass Spectrometry Lab
Last NameUlmer
First NameCandice
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Phone(352) 392-0515
Submit Date2015-09-15
Num Groups4
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Uploaded File Size2.2 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Candice Ulmer Candice Ulmer application/zip

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Combined analysis:

Analysis ID AN000384
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS
Column Supelco Analytical Titan C18
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Units Peak Area


Chromatography ID:CH000276
Chromatography Summary:Reversed Phase
Methods Filename:m_lipid_pos_switch_100
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Supelco Analytical Titan C18
Column Pressure:1241 bar (max)
Column Temperature:30 C
Flow Gradient:32% B at 0 min, 40% B at 1 min, a hold at 40% B until 1.5 min, 45% B at 4 min, 50% B at 5 min, 60% B at 8 min, 70% B at 11 min, and 80% B at 14 min
Flow Rate:0.5 mL/min
Injection Temperature:4 C
Sample Injection:2 μL
Solvent A:40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate
Analytical Time:21 min
Randomization Order:3,10,12,11,8,6,5,4,7,9,2,1
Chromatography Type:Reversed phase