Summary of Study ST000246
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000246 |
Study Title | Lipid Extraction Efficiency Comparison |
Study Type | Lipid Extraction Efficiency Comparison |
Study Summary | Aliquots of Jurkat T-lymphocyte cells were extracted using the Folch or Matyash method at different total volumes and run in triplicate to calculate the extraction efficiency for each method. |
Institute | University of Florida |
Department | Dept. of Chemistry/SECIM |
Laboratory | Biomedical Mass Spectrometry Lab |
Last Name | Ulmer |
First Name | Candice |
Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
czulmer@chem.ufl.edu | |
Phone | (352) 392-0515 |
Submit Date | 2015-09-15 |
Num Groups | 4 |
Total Subjects | 12 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Uploaded File Size | 2.2 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
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Collection:
Collection ID: | CO000257 |
Collection Summary: | - |
Sample Type: | Cells - Jurkat, Clone E6-1 |
Collection Method: | Centrifugation |
Collection Frequency: | 1200 rpm |
Collection Duration: | 5 min |
Volumeoramount Collected: | 10 mL of cell suspension |
Storage Conditions: | -80°C |
Tissue Cell Quantity Taken: | 1e6 cells |