Summary of Study ST000246
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000246 |
Study Title | Lipid Extraction Efficiency Comparison |
Study Type | Lipid Extraction Efficiency Comparison |
Study Summary | Aliquots of Jurkat T-lymphocyte cells were extracted using the Folch or Matyash method at different total volumes and run in triplicate to calculate the extraction efficiency for each method. |
Institute | University of Florida |
Department | Dept. of Chemistry/SECIM |
Laboratory | Biomedical Mass Spectrometry Lab |
Last Name | Ulmer |
First Name | Candice |
Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
czulmer@chem.ufl.edu | |
Phone | (352) 392-0515 |
Submit Date | 2015-09-15 |
Num Groups | 4 |
Total Subjects | 12 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Uploaded File Size | 2.2 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
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Project:
Project ID: | PR000125 |
Project DOI: | doi: 10.21228/M8DW2B |
Project Title: | Mammalian Suspension-Cultured Cellular Metabolomics Workflow |
Project Type: | Metabolomics, Lipidomics, Untargeted |
Project Summary: | A workflow was optimized for the sample preparation of a single suspension-cultured cell pellet for both metabolomics and lipidomics analysis. Jurkat T-lymphocyte cells were washed with various rinsing solutions and the lipids extracted using different lipid extraction protocols to allow for the most reproducible and quantitative method. |
Institute: | University of Florida |
Department: | Dept. of Chemistry/SECIM |
Laboratory: | Biomedical Mass Spectrometry Lab |
Last Name: | Yost |
First Name: | Richard |
Address: | P.O. Box 117200, Gainesville, FL 32611 |
Email: | ryost@aa.ufl.edu |
Phone: | (352) 392-0515 |
Funding Source: | JDRF Research Grant |