Summary of Study ST000246

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.

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Study IDST000246
Study TitleLipid Extraction Efficiency Comparison
Study TypeLipid Extraction Efficiency Comparison
Study SummaryAliquots of Jurkat T-lymphocyte cells were extracted using the Folch or Matyash method at different total volumes and run in triplicate to calculate the extraction efficiency for each method.
Institute
University of Florida
DepartmentDept. of Chemistry/SECIM
LaboratoryBiomedical Mass Spectrometry Lab
Last NameUlmer
First NameCandice
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailczulmer@chem.ufl.edu
Phone(352) 392-0515
Submit Date2015-09-15
Num Groups4
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Uploaded File Size2.2 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Candice Ulmer Candice Ulmer
https://dx.doi.org/10.21228/M8DW2B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000271
Sampleprep Summary:Cells were extracted using either the Folch or Matyash method. The total volume of MeOH in each extraction method was varied (0.5 mL or 2.0 mL) while the ratio of MeOH to the other solvents was maintained.
Sampleprep Protocol Filename:SOP_LipidExtractionProtocol.pdf
Processing Method:cell lysis
Processing Storage Conditions:on ice
Extraction Method:Folch or Matyash lipid extraction method
Extract Concentration Dilution:1e6 cells were extracted with the solvent systems explained in the Sample Prep Summary
Sample Resuspension:100 μL of IPA
Sample Spiking:internal standards: lysophosphatidylcholine (LPC 17:0 and LPC 19:0), phosphatidylcholine (PC 17:0/17:0 and PC 19:0/19:0), phosphatidylethanolamine (PE 15:0/15:0 and PE 17:0/17:0), phosphatidylserine (PS 14:0/14:0 and PS 17:0/17:0), and phosphatidylglycerol (PG 14:0/14:0 and PG 17:0/17:0), triacylglyceride (TG 15:0/15:0/15:0 and TG 17:0/17:0/17:0)
Cell Type:Jurkat, Clone E6-1
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