Summary of Study ST000246
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000246 |
Study Title | Lipid Extraction Efficiency Comparison |
Study Type | Lipid Extraction Efficiency Comparison |
Study Summary | Aliquots of Jurkat T-lymphocyte cells were extracted using the Folch or Matyash method at different total volumes and run in triplicate to calculate the extraction efficiency for each method. |
Institute | University of Florida |
Department | Dept. of Chemistry/SECIM |
Laboratory | Biomedical Mass Spectrometry Lab |
Last Name | Ulmer |
First Name | Candice |
Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
czulmer@chem.ufl.edu | |
Phone | (352) 392-0515 |
Submit Date | 2015-09-15 |
Num Groups | 4 |
Total Subjects | 12 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Uploaded File Size | 2.2 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000271 |
Sampleprep Summary: | Cells were extracted using either the Folch or Matyash method. The total volume of MeOH in each extraction method was varied (0.5 mL or 2.0 mL) while the ratio of MeOH to the other solvents was maintained. |
Sampleprep Protocol Filename: | SOP_LipidExtractionProtocol.pdf |
Processing Method: | cell lysis |
Processing Storage Conditions: | on ice |
Extraction Method: | Folch or Matyash lipid extraction method |
Extract Concentration Dilution: | 1e6 cells were extracted with the solvent systems explained in the Sample Prep Summary |
Sample Resuspension: | 100 μL of IPA |
Sample Spiking: | internal standards: lysophosphatidylcholine (LPC 17:0 and LPC 19:0), phosphatidylcholine (PC 17:0/17:0 and PC 19:0/19:0), phosphatidylethanolamine (PE 15:0/15:0 and PE 17:0/17:0), phosphatidylserine (PS 14:0/14:0 and PS 17:0/17:0), and phosphatidylglycerol (PG 14:0/14:0 and PG 17:0/17:0), triacylglyceride (TG 15:0/15:0/15:0 and TG 17:0/17:0/17:0) |
Cell Type: | Jurkat, Clone E6-1 |