Summary of Study ST000337
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000269. The data can be accessed directly via it's Project DOI: 10.21228/M81307 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000337 |
Study Title | Metabolomics Approach to Allograft Assessment in Liver Transplantation |
Study Type | Retrospective analysis of biobanked liver tissue from organ donors |
Study Summary | This pilot study is designed to apply several types of metabolomic analysis for liver allograft assessment with the aim of identifying candidate biomarkers for allograft function and to develop a methodology that could be applied to larger scale studies. The three main categories of metabolomic analysis are reflected in each of the specific aims, including a targeted profiling of central carbon metabolism, open lipidomic and metabolomic profiling for hypothesis generation and MALDI-IMS for tissue-based spatial analysis. Each of these approaches offers potential benefits that could be optimized in a protocol for larger scale studies depending the results of the pilot investigations. |
Institute | Ochsner Multi-Organ Transplant Institute |
Department | SECIM |
Laboratory | Abdominal Organ Transplantation |
Last Name | Seal |
First Name | John |
Address | 1514 Jefferson Highway, New Orleans, LA 70121 |
John.Seal@ochsner.org | |
Phone | 504-232-4253 |
Submit Date | 2016-01-17 |
Num Groups | 3 |
Total Subjects | Targeted analysis and open profiling: Control group - standard criteria donor (N=15); Experimental group - donation-after-cardiac-death donors (N=10). MALDI-IMS - standard criteria donor (N=10) |
Raw Data File Type(s) | raw(Thermo), d |
Analysis Type Detail | LC-MS |
Release Date | 2017-01-17 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000544 | AN000545 | AN000546 | AN000547 | AN000548 |
---|---|---|---|---|---|
Analysis type | MS | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Thermo Dionex Ultimate 3000 |
Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (50 x 2mm,1.7um) | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ | Thermo Quantiva QQQ |
Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
Units | nmol/mg dry powder | pmol/mg dry powder | nmol/mg dry powder | nmol/mg dry powder | nmol/mg dry powder |
Chromatography:
Chromatography ID: | CH000381 |
Chromatography Summary: | Amino acid assay |
Methods Filename: | SB_AA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
Column Temperature: | 55 |
Flow Rate: | 0.7 mL/min |
Solvent A: | AccQ·Tag Eluent A |
Solvent B: | accQ·Tag Eluent B |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000382 |
Chromatography Summary: | CoA assay |
Methods Filename: | SB_CoA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000383 |
Chromatography Summary: | NAD-ATP assay |
Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000384 |
Chromatography Summary: | NADH assay |
Methods Filename: | SB_NADH_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 30 |
Flow Rate: | 0.54 mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 6 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000385 |
Chromatography Summary: | Organic acid assay |
Methods Filename: | SB_OA_Assay.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 45 |
Flow Rate: | 0.3 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |