Summary of Study ST000337
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000269. The data can be accessed directly via it's Project DOI: 10.21228/M81307 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000337 |
Study Title | Metabolomics Approach to Allograft Assessment in Liver Transplantation |
Study Type | Retrospective analysis of biobanked liver tissue from organ donors |
Study Summary | This pilot study is designed to apply several types of metabolomic analysis for liver allograft assessment with the aim of identifying candidate biomarkers for allograft function and to develop a methodology that could be applied to larger scale studies. The three main categories of metabolomic analysis are reflected in each of the specific aims, including a targeted profiling of central carbon metabolism, open lipidomic and metabolomic profiling for hypothesis generation and MALDI-IMS for tissue-based spatial analysis. Each of these approaches offers potential benefits that could be optimized in a protocol for larger scale studies depending the results of the pilot investigations. |
Institute | Ochsner Multi-Organ Transplant Institute |
Department | SECIM |
Laboratory | Abdominal Organ Transplantation |
Last Name | Seal |
First Name | John |
Address | 1514 Jefferson Highway, New Orleans, LA 70121 |
John.Seal@ochsner.org | |
Phone | 504-232-4253 |
Submit Date | 2016-01-17 |
Num Groups | 3 |
Total Subjects | Targeted analysis and open profiling: Control group - standard criteria donor (N=15); Experimental group - donation-after-cardiac-death donors (N=10). MALDI-IMS - standard criteria donor (N=10) |
Raw Data File Type(s) | raw(Thermo), d |
Analysis Type Detail | LC-MS |
Release Date | 2017-01-17 |
Release Version | 1 |
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Collection:
Collection ID: | CO000351 |
Collection Summary: | A wedge biopsy of the right lobe of the liver is routinely obtained prior to transplantation. The sample is immediately flash frozen in the operating room and subsequently transferred to Ochsner Biobank for sample storage. |
Sample Type: | Liver Tissue |
Collection Method: | Open biopsy during cold storage preservation of organ for transplant |
Collection Location: | Operating room of liver transplant during |
Collection Frequency: | Single sample prior to transplant |
Collection Duration: | Not applicable |
Collection Time: | Not applicable |
Volumeoramount Collected: | ~50mg |
Storage Conditions: | -180 degree C, liquid nitrogen vapor phase |
Collection Vials: | ThermoScientific CryoTube vials, 1.8mL |
Storage Vials: | ThermoScientific CryoTube vials, 1.8mL |
Collection Tube Temp: | -70 degree liguid nitrogen flash freezing |
Additives: | None |
Blood Serum Or Plasma: | N/A |
Tissue Cell Identification: | N/A |
Tissue Cell Quantity Taken: | N/A |