Summary of Study ST001852
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001168. The data can be accessed directly via it's Project DOI: 10.21228/M8QT2F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001852 |
| Study Title | Screening of unique bile acid metabolizing bacteria |
| Study Summary | We incubated individual bacterial strains at pH 7 or pH 9 with either CDCA, LCA, or 3-oxo-Δ4-LCA as starting substrates. Culture supernatants were collected after 48 hours and 14 bile acids were measured by targeted metabolomics. |
| Institute | Keio University School of Medicine |
| Department | Dept of Microbiology and Immunology |
| Last Name | Koji |
| First Name | Atarashi |
| Address | 35 Shinanomachi, Shinjuku-ku, Tokyo, JAPAN |
| kojiatarashi@keio.jp | |
| Phone | 0353633769 |
| Submit Date | 2021-06-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | lcd |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-07-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003000 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu CBM20A |
| Column | InertSustain C18 |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu LCMS-8040 |
| Ion Mode | UNSPECIFIED |
| Units | uM |
Chromatography:
| Chromatography ID: | CH002225 |
| Chromatography Summary: | 2 μL of the solution was injected to LC/ESI-MS/MS (LC-MS8040 tandem mass spectrometer, equipped with an ESI probe and Nexera X2 ultra-high-pressure liquid chromatography system; Shimadzu). A separation column, InertSustain C18 (150 mm × 2.1 mm ID, 2 μm particle size; GL Sciences Inc.), was utilized at 40 °C. Mixture A (10 mM ammonium acetate, 0.01% formic acid, and 20% acetonitrile) and mixture B (30% acetonitrile and 70% methanol) were used as the eluent, and the separation was carried out by linear gradient elution at a flow rate of 0.2 mL/min. The mobile phase composition was gradually changed as follows: Mixture A:B (80:20, v/v) for 0.1 min, (48:52, v/v) for 0.1-1 min, (30:70, v/v) for 1-27 min, (0:100, v/v) for 27-27.1 min, (0:100, v/v) for 27.1-33 min, (80:20, v/v) for 33-33.1 min, and (80:20 v/v) for 33.1-83 min. The total run time was 38 min. |
| Instrument Name: | Shimadzu CBM20A |
| Column Name: | InertSustain C18 |
| Column Temperature: | 40 |
| Flow Gradient: | The mobile phase composition was gradually changed as follows: Mixture A:B (80:20, v/v) for 0.1 min, (48:52, v/v) for 0.1-1 min, (30:70, v/v) for 1-27 min, (0:100, v/v) for 27-27.1 min, (0:100, v/v) for 27.1-33 min, (80:20, v/v) for 33-33.1 min, and (80:20 v/v) for 33.1-83 min. The total run time was 38 min. |
| Flow Rate: | 0.2ml/min |
| Solvent A: | 20% acetonitrile/80% water; 0.01% formic acid; 10mM ammonium acetate |
| Solvent B: | 30% acetonitrile/70% methanol |
| Chromatography Type: | Reversed phase |
