Summary of Study ST001852
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001168. The data can be accessed directly via it's Project DOI: 10.21228/M8QT2F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001852 |
| Study Title | Screening of unique bile acid metabolizing bacteria |
| Study Summary | We incubated individual bacterial strains at pH 7 or pH 9 with either CDCA, LCA, or 3-oxo-Δ4-LCA as starting substrates. Culture supernatants were collected after 48 hours and 14 bile acids were measured by targeted metabolomics. |
| Institute | Keio University School of Medicine |
| Department | Dept of Microbiology and Immunology |
| Last Name | Koji |
| First Name | Atarashi |
| Address | 35 Shinanomachi, Shinjuku-ku, Tokyo, JAPAN |
| kojiatarashi@keio.jp | |
| Phone | 0353633769 |
| Submit Date | 2021-06-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | lcd |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-07-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003000 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu CBM20A |
| Column | InertSustain C18 |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu LCMS-8040 |
| Ion Mode | UNSPECIFIED |
| Units | uM |
MS:
| MS ID: | MS002789 |
| Analysis ID: | AN003000 |
| Instrument Name: | Shimadzu LCMS-8040 |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The following MS parameters were used: spray voltage; 3,000V, heating block temperature; 400°C, nebulizing gas flow; 3 L/min, drying gas flow; 15 L/min, interface temperature 300°C, collision gas (argon) pressure; 230 kPa, collision energy; negative (11 to -35 eV); and positive (-16 to -19 eV) ion modes. Samples were analysed and quantified using LabSolutions Insight LC-MS software (Shimadzu). |
| Ion Mode: | UNSPECIFIED |
