Summary of Study ST001852
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001168. The data can be accessed directly via it's Project DOI: 10.21228/M8QT2F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001852 |
| Study Title | Screening of unique bile acid metabolizing bacteria |
| Study Summary | We incubated individual bacterial strains at pH 7 or pH 9 with either CDCA, LCA, or 3-oxo-Δ4-LCA as starting substrates. Culture supernatants were collected after 48 hours and 14 bile acids were measured by targeted metabolomics. |
| Institute | Keio University School of Medicine |
| Department | Dept of Microbiology and Immunology |
| Last Name | Koji |
| First Name | Atarashi |
| Address | 35 Shinanomachi, Shinjuku-ku, Tokyo, JAPAN |
| kojiatarashi@keio.jp | |
| Phone | 0353633769 |
| Submit Date | 2021-06-24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | lcd |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-07-07 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR001941 |
| Treatment Summary: | Under anaerobic conditions, isolated bacteria strains were cultured together with 50 μM of CDCA, 3-oxo-Δ4-LCA, or LCA to screen for their bile acid metabolism in a 96-deep well plate covered with a gas-permeable membrane. 20 μL of bacterial culture in exponential to stationary phase was inoculated into 1 mL of Wilkins-Chalgren Anaerobe (WCA) media adjusted to pH 7 (using MOPS buffer solution) or pH 9 (TAPS buffer solution). |
