Summary of Study ST000817
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000582. The data can be accessed directly via it's Project DOI: 10.21228/M8FD4K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000817 |
| Study Title | Dynamics of metabolism, proliferation and differentiation in Toxoplasma gondii mutants deficient in glycolysis and gluconeogenesis |
| Study Type | Time course 13C labeling of Toxoplasma gondii using glucose and glutamine |
| Study Summary | The focus of this study was to profile the metabolic fates of glucose and glutamine in wild type, glycolytic mutant (hexokinase KO) and gluconeogenesis mutant (PEPCK1 KO) tachyzoite stage T. gondii parasites. 13C6-glucose and 13C5-15N1-glutamine were used as metabolic tracers. Metabolites from glycolysis, pentosephosphate pathway and Kerbs cycle were identified and their isotopomer abundance was quantified in order to visualize metabolic flux across the indicated pathways. A time course (from 0 minutes to 120 minutes) analysis was carried out in the labeling experiments. Here, host cell free extracellular tachyzoite stage T. gondii parasites were used. |
| Institute | CSIR-National Chemical Laboratory |
| Department | Biochemical Sciences Division |
| Laboratory | Molecular Parasitology Laboratory |
| Last Name | Shanmugam |
| First Name | Dhanasekaran |
| Address | Dr. Homi Bhabha Road, Pune, 411008, Maharashtra, India |
| d.shanmugam@ncl.res.in | |
| Phone | +91-20-25902719 |
| Submit Date | 2017-07-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-10-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO000837 |
| Collection Summary: | Extracellular tachyzoite stage Toxoplasma gondii parasites incubated in either +13C6-glucose or +13C5-15N2-glutamine containing DMEM medium for 0, 5, 10, 30, 60 & 120 minutes were collected by centrifugation and immediately extracted with chilled acetonitrile:water (80:20) mixture and further process for LC-MS analysis. |
| Sample Type: | Cells |
| Collection Method: | Centrifuging cell suspension at 5000 rpm for 5 min at 4 deg. Cel. |
| Collection Time: | At 0, 5, 10, 30, 60 & 120 minutes post labeling with either +13C6-glucose or +13C5-15N2-glutamine. |
| Volumeoramount Collected: | 10^8 cells per sample |
| Storage Conditions: | -80 deg. Cel. Freezer post extraction until LC-MS analysis can be carried out. |
| Collection Vials: | 1.5 ml capped tubes |
| Storage Vials: | 1.5 ml capped tubes |
| Collection Tube Temp: | 4 deg. Cel. |
