Summary of Study ST000817
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000582. The data can be accessed directly via it's Project DOI: 10.21228/M8FD4K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000817 |
Study Title | Dynamics of metabolism, proliferation and differentiation in Toxoplasma gondii mutants deficient in glycolysis and gluconeogenesis |
Study Type | Time course 13C labeling of Toxoplasma gondii using glucose and glutamine |
Study Summary | The focus of this study was to profile the metabolic fates of glucose and glutamine in wild type, glycolytic mutant (hexokinase KO) and gluconeogenesis mutant (PEPCK1 KO) tachyzoite stage T. gondii parasites. 13C6-glucose and 13C5-15N1-glutamine were used as metabolic tracers. Metabolites from glycolysis, pentosephosphate pathway and Kerbs cycle were identified and their isotopomer abundance was quantified in order to visualize metabolic flux across the indicated pathways. A time course (from 0 minutes to 120 minutes) analysis was carried out in the labeling experiments. Here, host cell free extracellular tachyzoite stage T. gondii parasites were used. |
Institute | CSIR-National Chemical Laboratory |
Department | Biochemical Sciences Division |
Laboratory | Molecular Parasitology Laboratory |
Last Name | Shanmugam |
First Name | Dhanasekaran |
Address | Dr. Homi Bhabha Road, Pune, 411008, Maharashtra, India |
d.shanmugam@ncl.res.in | |
Phone | +91-20-25902719 |
Submit Date | 2017-07-22 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-11 |
Release Version | 1 |
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Subject:
Subject ID: | SU000843 |
Subject Type: | Cells (protozoan parasite) |
Subject Species: | Toxoplasma gondii |
Taxonomy ID: | 5811 |
Genotype Strain: | RH (Type I strain) |
Cell Biosource Or Supplier: | ATCC |
Subject Comments: | Asexual stage (Tachyzoite) |
Cell Passage Number: | In continuous culture |
Cell Counts: | 10^8 cells per sample |
Species Group: | Microorganism |