Summary of Study ST000817
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000582. The data can be accessed directly via it's Project DOI: 10.21228/M8FD4K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000817 |
| Study Title | Dynamics of metabolism, proliferation and differentiation in Toxoplasma gondii mutants deficient in glycolysis and gluconeogenesis |
| Study Type | Time course 13C labeling of Toxoplasma gondii using glucose and glutamine |
| Study Summary | The focus of this study was to profile the metabolic fates of glucose and glutamine in wild type, glycolytic mutant (hexokinase KO) and gluconeogenesis mutant (PEPCK1 KO) tachyzoite stage T. gondii parasites. 13C6-glucose and 13C5-15N1-glutamine were used as metabolic tracers. Metabolites from glycolysis, pentosephosphate pathway and Kerbs cycle were identified and their isotopomer abundance was quantified in order to visualize metabolic flux across the indicated pathways. A time course (from 0 minutes to 120 minutes) analysis was carried out in the labeling experiments. Here, host cell free extracellular tachyzoite stage T. gondii parasites were used. |
| Institute | CSIR-National Chemical Laboratory |
| Department | Biochemical Sciences Division |
| Laboratory | Molecular Parasitology Laboratory |
| Last Name | Shanmugam |
| First Name | Dhanasekaran |
| Address | Dr. Homi Bhabha Road, Pune, 411008, Maharashtra, India |
| d.shanmugam@ncl.res.in | |
| Phone | +91-20-25902719 |
| Submit Date | 2017-07-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-10-11 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000857 |
| Treatment Summary: | Tachyzoite stage Toxoplasma gondii parasites were freshly isolated from infected Human Foreskin Fibroblast monolayers and the extracellular parasites were incubated in DMEM medium supplemented with either +13C6-glucose or +13C5-15N2-glutamine. Treatment with labeled substrates was done for 0, 5, 10, 30, 60 & 120 minutes before harvesting and processing the cells for LC-MS analysis. |
| Cell Storage: | Liquid nitrogen cell storage system |
| Cell Growth Container: | Standard incubator maintaining 37 deg. Cel. & 5% CO2 |
| Cell Media: | DMEM minus glucose medium (cat. No. 11966; Gibco, ThermoFisher Scientific) supplemented with +13C6-glucose. DMEM minus glutamine medium (cat. No. 11960; Gibco, ThermoFisher Scientific) supplemented with +13C5-15N2-glutamine. |
