Summary of Study ST000817

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000582. The data can be accessed directly via it's Project DOI: 10.21228/M8FD4K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000817
Study TitleDynamics of metabolism, proliferation and differentiation in Toxoplasma gondii mutants deficient in glycolysis and gluconeogenesis
Study TypeTime course 13C labeling of Toxoplasma gondii using glucose and glutamine
Study SummaryThe focus of this study was to profile the metabolic fates of glucose and glutamine in wild type, glycolytic mutant (hexokinase KO) and gluconeogenesis mutant (PEPCK1 KO) tachyzoite stage T. gondii parasites. 13C6-glucose and 13C5-15N1-glutamine were used as metabolic tracers. Metabolites from glycolysis, pentosephosphate pathway and Kerbs cycle were identified and their isotopomer abundance was quantified in order to visualize metabolic flux across the indicated pathways. A time course (from 0 minutes to 120 minutes) analysis was carried out in the labeling experiments. Here, host cell free extracellular tachyzoite stage T. gondii parasites were used.
Institute
CSIR-National Chemical Laboratory
DepartmentBiochemical Sciences Division
LaboratoryMolecular Parasitology Laboratory
Last NameShanmugam
First NameDhanasekaran
AddressDr. Homi Bhabha Road, Pune, 411008, Maharashtra, India
Emaild.shanmugam@ncl.res.in
Phone+91-20-25902719
Submit Date2017-07-22
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2017-10-11
Release Version1
Dhanasekaran Shanmugam Dhanasekaran Shanmugam
https://dx.doi.org/10.21228/M8FD4K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cells (protozoan parasite); Subject species: Toxoplasma gondii (Factor headings shown in green)

mb_sample_id local_sample_id Cell line
SA045294Tgon_HKKO_13Cglucose_t060_AT. gondii RH HKKO
SA045295Tgon_HKKO_13Cglucose_t060_BT. gondii RH HKKO
SA045296Tgon_HKKO_13Cglucose_t120_BT. gondii RH HKKO
SA045297Tgon_HKKO_13Cglucose_t030_BT. gondii RH HKKO
SA045298Tgon_HKKO_13Cglucose_t120_AT. gondii RH HKKO
SA045299Tgon_HKKO_13Cglucose_t010_BT. gondii RH HKKO
SA045300Tgon_HKKO_13Cglucose_t000_BT. gondii RH HKKO
SA045301Tgon_HKKO_13Cglucose_t005_AT. gondii RH HKKO
SA045302Tgon_HKKO_13Cglucose_t010_AT. gondii RH HKKO
SA045303Tgon_HKKO_13Cglutamine_t000_AT. gondii RH HKKO
SA045304Tgon_HKKO_13Cglucose_t030_AT. gondii RH HKKO
SA045305Tgon_HKKO_13Cglutamine_t005_AT. gondii RH HKKO
SA045306Tgon_HKKO_13Cglutamine_t060_AT. gondii RH HKKO
SA045307Tgon_HKKO_13Cglutamine_t060_BT. gondii RH HKKO
SA045308Tgon_HKKO_13Cglutamine_t120_AT. gondii RH HKKO
SA045309Tgon_HKKO_13Cglutamine_t120_BT. gondii RH HKKO
SA045310Tgon_HKKO_13Cglutamine_t030_BT. gondii RH HKKO
SA045311Tgon_HKKO_13Cglutamine_t030_AT. gondii RH HKKO
SA045312Tgon_HKKO_13Cglucose_t000_AT. gondii RH HKKO
SA045313Tgon_HKKO_13Cglutamine_t005_BT. gondii RH HKKO
SA045314Tgon_HKKO_13Cglutamine_t010_AT. gondii RH HKKO
SA045315Tgon_HKKO_13Cglutamine_t010_BT. gondii RH HKKO
SA045316Tgon_HKKO_13Cglutamine_t000_BT. gondii RH HKKO
SA045317Tgon_HKKO_13Cglucose_t005_BT. gondii RH HKKO
SA045318Tgon_PEPCKKO_minusglucose_13Cglutamine_5T. gondii RH PEPCKKO
SA045319Tgon_PEPCKKO_minusglucose_13Cglutamine_6T. gondii RH PEPCKKO
SA045320Tgon_PEPCKKO_minusglucose_13Cglutamine_4T. gondii RH PEPCKKO
SA045321Tgon_RH_13Cglucose_t010_BT. gondii RH wt
SA045322Tgon_RH_13Cglucose_t010_AT. gondii RH wt
SA045323Tgon_RH_13Cglucose_t030_AT. gondii RH wt
SA045324Tgon_RH_13Cglucose_t030_BT. gondii RH wt
SA045325Tgon_RH_13Cglucose_t005_BT. gondii RH wt
SA045326Tgon_RH_13Cglucose_t060_AT. gondii RH wt
SA045327Tgon_PEPCKwt_minusglucose_13Cglutamine_1T. gondii RH wt
SA045328Tgon_RH_13Cglucose_t000_BT. gondii RH wt
SA045329Tgon_PEPCKwt_minusglucose_13Cglutamine_3T. gondii RH wt
SA045330Tgon_PEPCKwt_minusglucose_13Cglutamine_2T. gondii RH wt
SA045331Tgon_RH_13Cglucose_t060_BT. gondii RH wt
SA045332Tgon_RH_13Cglucose_t005_AT. gondii RH wt
SA045333Tgon_RH_13Cglucose_t120_AT. gondii RH wt
SA045334Tgon_RH_13Cglutamine_t030_BT. gondii RH wt
SA045335Tgon_RH_13Cglutamine_t030_AT. gondii RH wt
SA045336Tgon_RH_13Cglutamine_t060_AT. gondii RH wt
SA045337Tgon_RH_13Cglutamine_t060_BT. gondii RH wt
SA045338Tgon_RH_13Cglutamine_t120_AT. gondii RH wt
SA045339Tgon_RH_13Cglucose_t000_AT. gondii RH wt
SA045340Tgon_RH_13Cglutamine_t010_BT. gondii RH wt
SA045341Tgon_RH_13Cglutamine_t010_AT. gondii RH wt
SA045342Tgon_RH_13Cglutamine_t000_AT. gondii RH wt
SA045343Tgon_RH_13Cglucose_t120_BT. gondii RH wt
SA045344Tgon_RH_13Cglutamine_t000_BT. gondii RH wt
SA045345Tgon_RH_13Cglutamine_t005_AT. gondii RH wt
SA045346Tgon_RH_13Cglutamine_t005_BT. gondii RH wt
SA045347Tgon_RH_13Cglutamine_t120_BT. gondii RH wt
Showing results 1 to 54 of 54
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