Summary of Study ST002696

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001668. The data can be accessed directly via it's Project DOI: 10.21228/M83H7N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002696
Study TitleDeficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice
Study TypeMS Untargeted Lipidomics
Study SummaryGenetic association studies have linked ATP10A and closely related type IV P-type ATPases (P4-ATPases) to insulin resistance and vascular complications, such as atherosclerosis. In addition, prior studies of mice harboring large, overlapping chromosomal deletions implicated Atp10A in the development of diet-induced obesity and insulin resistance. Here, we generated gene-specific Atp10A knockout mice and show that Atp10A-/- mice fed a high-fat diet did not gain excess weight relative to wild-type littermates. However, Atp10A-/- mice displayed female-specific dyslipidemia characterized by elevated plasma triglycerides, free fatty acids and cholesterol, as well as altered VLDL and HDL properties. We also observed increased circulating levels of several sphingolipid species along with reduced levels of eicosanoids and bile acids. The Atp10A-/- mice also displayed hepatic insulin resistance without perturbations to whole-body glucose homeostasis. Thus, ATP10A has a sex-specific role in regulating plasma lipid composition and maintaining hepatic liver insulin sensitivity in mice.
Institute
Vanderbilt University
DepartmentChemistry
LaboratoryCenter for Innovative Technology
Last NameMay
First NameJody
Address2301 Vanderbilt Place, Nashville, TN, 37235, USA
Emailjody.c.may@vanderbilt.edu
Phone615-875-8438
Submit Date2023-04-20
Num Groups2
Total Subjects10
Publicationssubmitted
Raw Data AvailableYes
Raw Data File Type(s)d, mzML
Analysis Type DetailLC-MS
Release Date2023-05-24
Release Version1
Jody May Jody May
https://dx.doi.org/10.21228/M83H7N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA267002ko1-2pATP10A-knockout
SA267003ko3-1nATP10A-knockout
SA267004ko2-1nATP10A-knockout
SA267005ko2-2pATP10A-knockout
SA267006ko1-1nATP10A-knockout
SA267007ko3-2pATP10A-knockout
SA267008ko4-1nATP10A-knockout
SA267009ko5-2pATP10A-knockout
SA267010ko4-2pATP10A-knockout
SA267011ko1-2nATP10A-knockout
SA267012ko2-2nATP10A-knockout
SA267013ko3-2nATP10A-knockout
SA267014ko4-2nATP10A-knockout
SA267015ko5-2nATP10A-knockout
SA267016ko1-1pATP10A-knockout
SA267017ko2-1pATP10A-knockout
SA267018ko5-1pATP10A-knockout
SA267019ko4-1pATP10A-knockout
SA267020ko3-1pATP10A-knockout
SA267021ko5-1nATP10A-knockout
SA267022wt5-2nWild-type
SA267023wt4-2nWild-type
SA267024wt3-2nWild-type
SA267025wt2-2nWild-type
SA267026wt1-2nWild-type
SA267027wt1-1nWild-type
SA267028wt1-2pWild-type
SA267029wt2-2pWild-type
SA267030wt5-1pWild-type
SA267031wt4-1pWild-type
SA267032wt2-1pWild-type
SA267033wt3-1pWild-type
SA267034wt3-2pWild-type
SA267035wt4-2pWild-type
SA267036wt3-1nWild-type
SA267037wt4-1nWild-type
SA267038wt2-1nWild-type
SA267039wt1-1pWild-type
SA267040wt5-2pWild-type
SA267041wt5-1nWild-type
Showing results 1 to 40 of 40
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