Summary of Study ST001894
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001192. The data can be accessed directly via it's Project DOI: 10.21228/M8MX3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001894 |
| Study Title | Involvement of Mieap in cardiolipin metabolism (part II) |
| Study Summary | Mass spectrometric data of Cardiolipin in Mice kidney (Mieap-WT vs. Mieap-KO), and Mice liver (Mieap-WT vs. Mieap-KO) |
| Institute | National Cancer Center Japan Research Institute |
| Last Name | Ikari |
| First Name | Naoki |
| Address | 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan |
| nikari@ncc.go.jp | |
| Phone | +81-3-3542-2511 |
| Submit Date | 2021-07-30 |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-09 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003075 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UltiMate 3000 (Thermo Fisher Scientific) |
| Column | Waters XBridge C18 (150 x 1.0mm,3.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE |
| Units | pmol/mg |
MS:
| MS ID: | MS002862 |
| Analysis ID: | AN003075 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Analyses were performed on a LC/MS/MS system consisting of a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific) equipped with an electrospray ionization source, and an UltiMate 3000 system (Thermo Fisher Scientific). The lipid samples were separated on Waters X-Bridge C18 column (3.5 μm, 150 mm × 1.0 mm i.d.) at 40°C using a gradient solvent system as follows: mobile phase A (isopropanol/methanol/water (5:1:4, v/v/v) supplemented with 5 mM ammonium formate and 0.05% ammonium hydroxide (28% in water))/mobile phase B (isopropanol supplemented with 5 mM ammonium formate and 0.05% ammonium hydroxide (28% in water)) ratios of 60%/40% (0 min), 40%/60% (1 min), 20%/80% (9 min), 5%/95% (11-30 min), 95%/5% (31-35 min) and 60%/40% (45 min). Flow rate was 25 μL/min. The source and ion transfer parameters applied were as followed: spray voltage 3.0 kV. For negative ionization modes, the sheath gas and the capillary temperature were maintained at 60 and 320 °C, respectively. The Orbitrap mass analyzer was operated at a resolving power of 70,000 in full-scan mode (scan range: 200–1800 m/z; automatic gain control (AGC) target:3e6) and of 35,000 in the Top 20 data-dependent MS2 mode (stepped normalized collision energy: 20, 30 and 40; isolation window: 4.0 m/z; AGC target: 1e5). Identification of CL molecular species was performed using with LipidSearch4.2 software (Mitsui knowledge industry). |
| Ion Mode: | NEGATIVE |
