Summary of Study ST001894
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001192. The data can be accessed directly via it's Project DOI: 10.21228/M8MX3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001894 |
Study Title | Involvement of Mieap in cardiolipin metabolism (part II) |
Study Summary | Mass spectrometric data of Cardiolipin in Mice kidney (Mieap-WT vs. Mieap-KO), and Mice liver (Mieap-WT vs. Mieap-KO) |
Institute | National Cancer Center Japan Research Institute |
Last Name | Ikari |
First Name | Naoki |
Address | 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan |
nikari@ncc.go.jp | |
Phone | +81-3-3542-2511 |
Submit Date | 2021-07-30 |
Analysis Type Detail | LC-MS |
Release Date | 2021-08-09 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001978 |
Sampleprep Summary: | Total lipids were extracted from samples using the Bligh-Dyer method. An aliquot of the organic phase was added to an equal volume of methanol before being loaded onto a DEAE-cellulose column (Wako Chemical) pre-equilibrated with chloroform. After successive washes with chloroform/methanol (1:1, v/v), acidic phospholipids were eluted with chloroform/methanol/HCl/water (12:12:1:1, v/v), followed by evaporation to dryness to yield a residue was soluble in methanol. |
Extraction Method: | the Bligh-Dyer method |