Summary of Study ST000104
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000096. The data can be accessed directly via it's Project DOI: 10.21228/M8NK5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000104 |
Study Title | Factors for Epigenetic Silencing of Lung Cancer Genes |
Study Type | Metabolomic analysis of Plasma |
Study Summary | To test the hypothesis that the propensity for silencing of tumor suppressor genes in the respiratory epithelium of chronic smokers by promoter hypermethylation is influenced by sequence variations that modify the activity of genes and microRNAÕs that directly or indirectly influence de novo methylation and chromatin remodeling. |
Institute | University of North Carolina |
Department | Discovery Science Technology |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2014-09-29 |
Num Groups | 3 |
Total Subjects | 69 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Uploaded File Size | 36 M |
Analysis Type Detail | NMR |
Release Date | 2016-06-18 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000120 |
Sampleprep Summary: | Aliquots of plasma samples (630 µL) were transferred into labeled tubes for experimental samples. Pooled high methylatiion, low methylation and combined plasma samples were prepared to serve as external QC samples. For all samples 70 µL of 10 mM formate solution (chemical shift indicator) in 0.9% saline in D2O with 0.02% NaN3 was added to each tube and were vortexed for 30 sec then centrifuged at 12000 rcf for 5 min. A 600 µL aliquot of the supernatant was transferred into 5 mm NMR tubes, which were kept on ice until data acquisition. |
Processing Storage Conditions: | 4°C |
Extract Storage: | -80C |
Sample Resuspension: | D2O Saline |
Sample Spiking: | formate |