Summary of Study ST000881

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000611. The data can be accessed directly via it's Project DOI: 10.21228/M8XQ41 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000881
Study TitleTargeted metabolomics data of leprosy patients with type 1 reaction
Study TypeTargeted MS/MS analysis in retrospective serum samples of leprosy patients with type 1 reaction
Study SummaryTargeted metabolomics-based analyses of pooled sera from 7 patients with T1R were conducted via LC-MS/MS.
Institute
Colorado State University
DepartmentDepartment of Microbiology, Immunology, and Pathology
LaboratoryBelisle
Last NameSilva
First NameCarlos
AddressRampart Road, 1682 Campus Delivery
Emailcadriano@rams.colostate.edu
Phone9702154962
Submit Date2017-08-02
Num Groups2
Total Subjects16
Num Males5
Num Females11
Study Comments2 groups (first group was leprosy patients with type 1 reaction, N=9; and second group was leprosy patients without type 1 reaction, N=7) 5 males (3 were leprosy patients with type 1 reaction and 2 were leprosy patients without type 1 reaction), 11 females (4 were leprosy patients with type 1 reaction and 7 were leprosy patients without type 1 reaction). For this study we used retrospective sera samples.
PublicationsJ Infect Dis. 2017 Feb 1;215(3):431-439. doi: 10.1093/infdis/jiw541.
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-08-02
Release Version1
Carlos Silva Carlos Silva
https://dx.doi.org/10.21228/M8XQ41
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000922
Sampleprep Summary:Serum samples (7 serum samples from leprosy patients with T1R) were pooled prior the extraction. These pooled serum samples were incubated with 3 volumes of cold methanol (100%) for 1 hour at -20C. After this incubation, the samples were centrifuged for 30 minutes at 18,000 xg at 4C to clear the supernatant. The supernatant was then transferred and dried under vacuum. Samples were re-suspended in 80 μl of 50% methanol. Prior to the injections on the LC-MS instrument, the samples were centrifuged for 30 minutes at 18,000 xg at 4oC.
Sampleprep Protocol ID:SP000100
Sampleprep Protocol Filename:060613_Methanol_Serum_Extraction
Sampleprep Protocol Comments:Use always nitrile gloves. Do not use gloves with powder. Use pipette tips with aerosol barrier.
Processing Method:Homogenization and solvent removal with Speed Vac
Processing Storage Conditions:on ice
Extraction Method:Cold Methanol extraction (3:1 methanol/water)
Extract Enrichment:no enrichment was performed
Extract Cleanup:Centrifugation
Extract Storage:-80C
Sample Resuspension:50% methanol
Sample Derivatization:no derivatization process was performed
Sample Spiking:Samples were spiked with the following commercial standards: arachidonic acid, docosahexaenoic acid, eicosapentaenoic acid and leukotriene B4
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