Summary of Study ST001848
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001165. The data can be accessed directly via it's Project DOI: 10.21228/M8411V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001848 |
| Study Title | Untargeted mass spectrometry reveals impact of high fat diet on peripheral amino acid regulation in a mouse model of Alzheimer’s Disease |
| Study Type | untargeted metabolomics analysis |
| Study Summary | APPSwe/PS1ΔE9 (APP/PSEN1) transgenic mice (to represent familial or early-onset AD) and wild-type litter mater controls were fed either a high-fat diet (HFD, 60% kcal from lard), low-fat diet (LFD, 10% kcal from lard) from 2 months of age, or reversal diet (REV, high-fat followed by low-fat from 9.5 months). |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | Codreanu |
| First Name | Simona |
| Address | 3009, Liberty Hills Drive |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6153477458 |
| Submit Date | 2021-06-03 |
| Num Groups | 6 |
| Total Subjects | 36 |
| Num Males | 18 |
| Num Females | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-08-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001931 |
| Sampleprep Summary: | For liver samples, frozen tissues were first individually pulverized using a mortar and pestle followed by lysing in 1 mL ice-cold lysis buffer ((1:1:2, ACN:MeOH:ammonium bicarbonate (0.1 M, pH 8.0) (LC-MS grade)). Individual samples were sonicated using a probe tip sonicator, 10 pulses, at 30% power, and cooled on ice between samples. A bicinchoninic acid (BCA) protein assay was used to determine the protein concentration for each individual sample and adjusted to a total amount of protein of 200 µg. For untargeted MS studies, SIL-ISs biotin-D2 and phenylalanine-D8 were added to each sample to assess sample processing steps (metabolite extraction and reconstitution). Following lysis and addition of SIL-ISs, protein precipitation was performed by adding 800 µL of ice-cold methanol (4x by volume). Samples were incubated at -80°C overnight. Following incubation, samples were centrifuged at 10,000 rpm for 10 min. The supernatant containing metabolites were dried in vacuo and metabolite extracts were stored frozen at -80 °C until ready to use. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Following lysis and standard addition, protein precipitation was performed by adding 800µL of ice-cold methanol (4x by volume). Samples were incubated at -80°C overnight. Following incubation, samples were centrifuged at 10,000 rpm for 10 min to eliminate proteins. The supernatants containing metabolites were dried via speed-vacuum. |
| Extract Storage: | -80℃ |
