Summary of Study ST000140
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000140 |
Study Title | Cell Rinsing Solution Comparison |
Study Type | Cell Rinsing Solution Comparison |
Study Summary | Aliquots of Jurkat T-lymphocyte cells were washed with 5 different rinsing solutions (0.3% amm. formate, 0.3% amm. acetate, 0.9% NaCl, 1 M PBS, 100 mM PBS) and ran in triplicate to monitor the effect of ion suppression on the electrospray ionization signal. |
Institute | University of Florida |
Department | Dept. of Chemistry/SECIM |
Laboratory | Biomedical Mass Spectrometry Lab |
Last Name | Ulmer |
First Name | Candice |
Address | P.O. Box 117200, Gainesville, FL 32611 |
czulmer@chem.ufl.edu | |
Phone | (352) 392-0515 |
Submit Date | 2015-03-02 |
Num Groups | 5 |
Total Subjects | 15 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Uploaded File Size | 3.1 G |
Analysis Type Detail | LC-MS |
Release Date | 2016-03-03 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000157 |
Sampleprep Summary: | 80% MeOH metabolite extraction |
Sampleprep Protocol ID: | SOP_PP_02 |
Sampleprep Protocol Filename: | SOP_PP_02_CZU.pdf |
Processing Method: | cell lysis |
Processing Storage Conditions: | on ice |
Extraction Method: | 80% MeOH |
Extract Concentration Dilution: | 1e6 cells/ 2 mL of extraction solvent |
Sample Resuspension: | 30 μL 0.1% formic acid in water |
Sample Spiking: | internal standards: (1) L-tryptophan-2,3,3-d3; (2) creatine-d3; (3) d-leucine-d10; (4) caffeine-d3 |
Cell Type: | Jurkat, Clone E6-1 |